Alginate scaffold for organ culture of cryopreserved-thawed human ovarian cortical follicles
نویسندگان
چکیده
منابع مشابه
I-6: Follicle Development in Culture of Frozen / Thawed Human Ovarian Tissue
The primary therapeutic goal for the oncology patient is survival. Recent advances in diagnoses and treatment of neoplasia have resulted in an ever-increasing number of patients being cured and resuming a normal life. However, recognized side effects of treatments used to eradicate malignancies are temporary or permanent sterility. The cryopreservation of ovarian tissue harvested before cancer ...
متن کاملEmbryonic fibroblasts enable the culture of primary ovarian follicles within alginate hydrogels.
Hydrogel-encapsulating culture systems support the consistent growth of ovarian follicles from various species, such as mouse, non-human primate, and human; however, further innovations are required for the efficient production of quality oocytes from early-stage follicles. In this report, we investigated the coculture of mouse ovarian follicles with mouse embryonic fibroblasts (MEFs), commonly...
متن کاملSurvival of human pre-antral follicles after cryopreservation of ovarian tissue, follicular isolation and in vitro culture in a calcium alginate matrix.
BACKGROUND Ovarian tissue cryopreservation is a promising technique to safeguard fertility in cancer patients. However, in some types of cancer, there is a risk of transmitting malignant cells present in the cryopreserved tissue. To avoid such a risk, pre-antral follicles could be isolated from ovarian tissue and grown in vitro. On the basis of this assumption, the aim of our study was to inves...
متن کاملIn vitro culture of ovarian follicles.
This review offers a practically oriented introduction to follicle culture in vitro, focusing on mouse follicles, but with reference to other species. The main principles of follicle growth are addressed, including the constraints of tissue culture, methods of follicle isolation, and techniques for individual and collective culture of intact follicles. Culture systems that support a spherical o...
متن کاملAn research on the isolation methods of frozen-thawed human ovarian preantral follicles.
OBJECTIVE To explore the effective isolation method for preantral follicles from human frozen-thawed ovarian tissue. METHODS The ovarian cortical tissue was frozen by direct cover vitrification (DCV). The frozen-thawed ovarian tissue was used for isolation of preantral follicles with collagenase combined with mechanical method and mechanical method alone, respectively. RESULTS 1. There was ...
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ژورنال
عنوان ژورنال: Journal of Assisted Reproduction and Genetics
سال: 2011
ISSN: 1058-0468,1573-7330
DOI: 10.1007/s10815-011-9605-x